Fast analysis using pillar array columns: Quantification of branched-chain α-keto acids in human plasma samples

Quantitative Determination of Branched-chain Amino Acids in Human Plasma Using Pressure-driven Liquid Chromatography with Pillar Array Columns

We have succeeded for the first time to quantify biological compounds in human plasma using pillar array columns. A fast and quantitative method for the determination of branched-chain amino acids (BCAAs) in human plasma using pressure-driven liquid chromatography on a chip was developed. BCAAs were firstly derivatized with 4-fluoro-7-nitro2,1,3-benzoxadiazole (NBD-F), and then analysis of NBD-...

analysis of ruin probability for insurance companies using markov chain

در این پایان نامه نشان داده ایم که چگونه می توان مدل ریسک بیمه ای اسپیرر اندرسون را به کمک زنجیره های مارکوف تعریف کرد. سپس به کمک روش های آنالیز ماتریسی احتمال برشکستگی ، میزان مازاد در هنگام برشکستگی و میزان کسری بودجه در زمان وقوع برشکستگی را محاسبه کرده ایم. هدف ما در این پایان نامه بسیار محاسباتی و کاربردی تر از روش های است که در گذشته برای محاسبه این احتمال ارائه شده است. در ابتدا ما نشا...

Microfabricated pillar array columns for liquid chromatography

Discovery of a metagenome-derived enzyme that produces branched-chain acyl-(acyl-carrier-protein)s from branched-chain α-keto acids.

Clones that confer the ability to produce N-acyl amino acids onto model cultured bacterial hosts are frequently identified in activity-based screens of soil DNA libraries. The N-acyl amino acids encoded by these clones are synthesized from acyl-(acylcarrier-protein)s (acyl-ACPs) and amino acids by a diverse group of bacterial enzymes referred to as N-acyl amino acid synthases (NASs). 2] The acy...

Fatty Acids: Branched-chain

Branched-chain fatty acids are common constituents of the lipids of bacteria and animals, although they are rarely found in the integral lipids of higher plants. Normally, the fatty acyl chain is saturated and the branch is a methyl-group. However, unsaturated branched-chain fatty acids are found in marine animals, and branches other than methyl may be present in microbial lipids. The most comm...